Enzymatic Oxidation of Leaf Alcohol by Allyl Alcohol Dehydrogenase from E. coli
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چکیده
منابع مشابه
Novel antioxidant role of alcohol dehydrogenase E from Escherichia coli.
Alcohol dehydrogenase E (AdhE) is an Fe-enzyme that, under anaerobic conditions, is involved in dissimilation of glucose. The enzyme is also present under aerobic conditions, its amount is about one-third and its activity is only one-tenth of the values observed under anaerobic conditions. Nevertheless, its function in the presence of oxygen remained ignored. The data presented in this paper le...
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The kinetics of oxidation of allyl alcohol by quinaldinium chlorochromate is investigated in 40 % acetic acid – water (v/v) medium. The order with respect to oxidant and hydrogen ion concentration is found to be one and fractional with respect to substrate. The rate of the reaction is decreases with the decrease in the dielectric constant of the medium. The rate of the reaction has negligible e...
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During a study of cyclic alcohol oxidation by a partially purified rat liver enzyme system (l), it was noted that ethanol was also oxidized by this preparation. Since the ratio of the rate of ethanol oxidation to the rate of cyclohexanol oxidation remained constant during purification, the possibility arose that the activity with cyclic substrates was due to the conventional alcohol dehydrogena...
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Alcohol dehydrogenase (alcohol: NAD oxidoreductase, E.C. 1.1.1.1.) mutants of Chinese hamster somatic cells were isolated as resistant to allyl alcohol (ALLR). The ALLR phenotypes of the mutant clones were reproducible with high fidelity and stable over long intervals of growth in the absence of the selecting drug. Several mutants, Adh-1, Adh-2, Adh-9 and Adh-13, resistant to allyl alcohol were...
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A potent new enzyme-antibody conjugate system for amplifying cytotoxicity was tested in a well-defined model of hapten [2,4,6-trinitrophenyl (TNP)]-substituted tumor cells (HEp2) and purified anti-hapten antibody. Brief treatment of TNP-HEp2 cells with low concentrations (0.05 to 0.74 micrograms/ml) of antihapten antibody-alcohol dehydrogenase conjugate (Ab-ADH) followed by culture in complemen...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1970
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.34.1574